Average of two biological replicates shown. A one-way within-sample ANOVA was performed and
demonstrated no significant difference in expression of nontargeted transcripts for truncated guides versus the full-length guide (P =
0.316). Correlation between samples and controls was also high (R ≥ 0.979). (b) Histograms showing the distribution of fold changes in
gene expression (activator/guide control). Genes were filtered to include only those with TPM > 1. Average of two biological replicates
shown.