Overexpression ofDNG701 in rice calli and plants led to marked hypomethylation of 45S rDNA and slight hypomethylation on centromeric repeat DNA and Ty1 retrotransposons, suggesting that DNG701 is targeted to these regions, and overexpression of DNG701 presumably has significant dose effect to excessively demethylate them (Fig. 3C and Fig. S9).