Theultracentrifugationwith
16 000r/minandreactiontime40minuteshaslowextractionefficiencyofEPSbutlittlecelldisruption.
Itcanserveasacontrolmethodtoestimatethecelldisruptioncausedbyothermethods.Theultrasonica-
tionwithfrequency40kHz,power50W andreactiontime20minuteshasnocompleteEPSextraction.It
canbeusedasapreliminarytreatmentinconjunctionwithanotherextractionmethod.