2.6. Structural determination of ol

2.6. Structural determination of oligosaccharides by NMR
Fifteen milligrams of each oligosaccharide was co-evaporated with D2O twice by lyophilisation and subsequently dissolved in 500 lL D2O containing 0.1 lL 4,4-dimethyl-4-silapentane-1-sul-phonic acid. The NMR spectra were recorded on a Bruker AVANCE III 600 spectroscope (Bruker, German). The 1-dimensional1H and 13C NMR spectra and 2-dimensional correlation spectroscopy
(COSY), total correlation spectroscopy (TOCSY) heteronuclear single quantum coherence (HSQC), heteronuclear multiple bond correlation (HMBC) and rotating-frame overhauser effect spectroscopy (ROESY) were all obtained at 600 MHz at 25 °C with sufficient acquisition time. The observed1H and13C chemical shifts were calibrated according to external standard 4,4-dimethyl-4-silapentane-1-sulfonic acid (0.00 ppm).

結果 (中文) 1:

2.6.低聚糖的 NMR 结构测定十五毫克每个低聚糖是共同蒸发与 D2O 两次由 lyophilisation 和随后溶解在 500 lL D2O 含有 0.1 lL 4,4-dimethyl-4-silapentane-1-sul-phonic 酸。核磁共振谱录上布鲁克阿旺 III 600 分光镜布鲁克德国）。1-dimensional1H 和 13c 核磁共振谱和二维相关红外光谱法(舒适)，共有相关光谱 （TOCSY） 异核单量子相干 （HSQC），异核多个键关联 （惠丰） 和旋转框架 overhauser 效应谱 （ROESY） 所有 600 mhz 获得在 25 ° C sufficient 采集时间。根据外部标准 4,4-dimethyl-4-silapentane-1-sulfonic 酸 (0.00 ppm) observed1H and13C 化学位移进行了刻度。

結果 (中文) 3:

2.6。通过核磁共振
十五毫克每寡糖结构测定寡糖共蒸发与D2O两次冻干和随后溶解在500会0.1会4,4-dimethyl-4-silapentane-1-sul-phonic酸D2O。核磁共振光谱记录在布鲁克皇冠III 600光谱仪（Bruker，德国）。的1-dimensional1h和13C核磁共振谱和二维相关谱（COSY），
总相关光谱（TOCSY）异核单量子相干（HSQC），异核多键相关（HMBC）和旋转框架的Overhauser效应谱（ROESY）均获得600兆赫25°C后fi有效采集时间。根据外部标准4,4-dimethyl-4-silapentane-1-sulfonic酸observed1h和13C化学位移（0 ppm）。