The FRET efficiency was strongly depe

The FRET efficiency was strongly dependent on the following conditions: (1) The emission band of the donor should overlap with the absorption band of the acceptor. (2) The distance between both species should be in close proximity (less than 10 nm).62 One of the advantages of C-dots as electron donor was that their emission wavelength can be manipulated by choosing different excitation wavelengths thus ensuring adaptability to any kinds of energy acceptors. For efficient FRET, the excitation wavelength of 350 nm was selected for excitation of C-dots (the energy donor) because the fluorescence at 350 nm excitation overlapped with the absorption band of R6G (the energy acceptor) to ensure the FRET process (Figure 1). Therefore, feasible energy transfer from C-dots to the rhodamine moiety was theoretically established. R6G-COOH was synthesized in two steps using R6G as the starting material (Scheme S1). Intermediate R6G-NH2 was prepared in a reaction between R6G and 1,2-ethylenediamine according to the previous report.58 R6G-NH2 was reacted with succinic acid to obtain R6G-COOH. The chemical structures of R6G-NH2 and R6G-COOH were confirmed by 1H NMR and FT-IR. R6G-COOH was designed to become immobilized on the surface of C-dots through the reaction between the
carboxylic acid group in R6G-COOH and the amino group in C-dots to form an amide linkage. Finally, rhodamine spirolactam was successfully incorporated in C-dots via the conventional DCC/DMAP method to obtain C-dots-R6G (Scheme 1). FT-IR spectra revealed the changes in the functional groups on the surface of C-dots upon surface modification (Figure S3). The as-prepared C-dots showed the characteristic bands at 1630 cm−1 for CC stretching, 2870 and 2940 cm−1 for C−H stretching, and a broad band at 3270 cm−1 corresponding to amine and hydroxyl groups. Upon surface modification with R6G-COOH, the characteristic absorption band of amide CO stretching at 1670 cm−1 was observed, which indicated that the rhodamine moieties were successfully incorporated in the C-dots. The amount of rhodamine moiety immobilized on the surface of C-dots was calculated from a calibrationcurveof rhodamine plotted with known concentrations of the open form of R6GCOOH at maximal absorption wavelength (λmax = 526 nm) using UV spectroscopy (Figure S4). It was found that the rhodamine moiety was introduced on the surface of C-dots at 5.80 × 10−2 mg/mg

結果 (繁體中文) 1:

FRET efficiency 是強烈地依賴于以下條件: (1) 螢光發射帶為捐助者應與承兌人的吸收帶重疊。（2） 這兩個物種之間的距離應在接近的接近度 (小於 10 nm).62 C 點作為電子供體的優點之一就是其發射波長可以通過選擇服務激發波長，從而確保對任何種類的能源受眾的適應性。為 efficient 別發愁，激發波長的 350 nm 入選勵磁的 C-點 （能源捐助） 因為在 350 nm 激發 fluorescence 與 R6G 吸收帶重疊 （能源承兌人） 以確保 FRET 過程 (圖 1)。因此，從理論上建立了從 C 點到羅丹明基團可行的能量傳遞。在使用 R6G 為起始原料 (計畫 S1) 的兩個步驟合成了 R6G-COOH。R6G 與 1，2-乙二胺根據以前的 report.58 R6G NH2 反應與丁二酸獲得 R6G-COOH 反應制得了中間 R6G NH2。R6G NH2 和 R6G-COOH 的化學結構是由 1 H NMR 和 IR 的牢固性R6G-COOH 旨在成為固定化方法對 C 點的表面之間的反應羧酸組 R6G-COOH 和 C 點的氨基基團形成醯胺鍵連接。最後，羅丹明產生成功通過常規的 DCC/DMAP 方法，以獲得 C-點-R6G (方案 1) 列入 C 點。傅立葉轉換紅外光譜顯示 C 點後表面 modification (圖 S3) 的表面官能團的變化。作為編寫 C 點顯示在 1630 cm − 1 為 CC 舒展、 2870年和 2940 cm − 1 為 C−H 舒展，波段和寬頻在 3270 cm − 1 對應于胺和羥基。後與 R6G-COOH 的表面 modification，醯胺的特徵吸收帶 CO 伸展在 1670 cm − 1 有人指出，這表明羅丹明基團被成功列入 C 點。從繪製用已知濃度的打開的表單的 R6GCOOH 在最大吸收波長 calibrationcurveof 羅丹羅丹明基團 C 點表面固定金額 (λ 最大 = 526 nm) 使用紫外分光光度法 (圖 S4)。它被發現羅丹明基團的介紹了 5.80 × 10 − 2 毫克/毫克 C 點的表面

結果 (繁體中文) 2:

FRET的ëFFI效率強烈依賴於下列條件：（1）供體的發射帶應與受體的吸收帶重疊。（2）這兩個物種之間的距離應在接近（小於10nm）0.62之一的C-點的優點為電子給體的是，他們的發射波長可以通過選擇二FF erent激發波長從而保證適應性任何被操縱種能量受體的。為電子FFI cient FRET，被選擇為350nm的激發波長用於C點（能量供體）的激發，因為在350nm處激發帶R6G（能量受體）的吸收帶重疊的FL熒光，以確保FRET過程（圖1 ）。因此，從C-點到羅丹明部分可行的能量傳遞理論上成立。R6G-COOH是在使用R6G作為原料（方案S1）兩步合成。中間R6G-NH 2在R6G和根據前述report.58 R6G-NH 2用琥珀酸，得到R6G-COOH反應的1,2-乙二胺的反應製備。R6G-NH2和R6G-COOH的化學結構是通過1H NMR和FT-IR Rmed指CON網絡連接。R6G-COOH被設計成為固定的C-點的表面上通過之間的反應
在R6G-COOH中的C-點的氨基羧酸基團和以形成酰胺鍵。最後，若丹明螺內酰胺中的C-點成功摻入通過傳統的DCC / DMAP的方法得到的C-點-R6G（流程1）。的FT-IR光譜顯示在官能團的C-點的表面上時表面改性音響陽離子（圖S3）的變化。所製備的C-點顯示特徵帶1630 -1為CC拉伸，2870 2940 -1為C-H伸縮，和寬的頻帶在3270厘米-1對應的胺和羥基。在用R6G-COOH 1670 -1拉伸酰胺CO的特性吸收帶表面改性音響陽離子觀察，表明若丹明部分分別在C-點成功引入。羅丹明部分的固定化的C-點的表面上的量從使用UV光譜（圖S4）與在最大吸收波長已知濃度R6GCOOH的開放形式的繪製一個calibrationcurveof羅丹明（λ最大= 526納米）來計算。人們發現，若丹明部分在5.80×10-2毫克/毫克C-點的表面上引入

結果 (繁體中文) 3:

跟你說話。