My thirst for knowledge is strong since I was young. I used to listen to my parents telling stories, and became curious about the natural world, thirsting for exploring it. When grew up, I was no longer content with those stories, so I began my own reading. I subscribed to We Love Science since elementary school, read lots of discoveries, wondering my own adventures someday. That’s the first time I had an interest in science.
Upon my entering Jilin University, I knew nothing about life science though. I chose to major in biotechnology just because I wanted to experience what I’ve read about in We Love Science when I was young. But at the end of my first semester, when I performed excellently in my academy and was given an opportunity to change my major, I hesitated. It was then I entered a class called Introduction to Life Science. During this general education course, the professor showed us the beauty and the charm of life science through his poetic description, passionate speech, vivid presentation. I was enchanted by this burgeoning field at once. When he explained to us how they finally managed to knockout some genes of the blue fox, changed its original white coat color, and decoded the cause of its death in ecstasy, I could feel his excitement, too, though I knew nothing about molecular genetics then. Through the course, I came to realize how wonderful life exists and brings about. At the end of this course, I made my decision to stay, for a keen fascination with life science. From then on, my journey was setting out to explore the colorful biology world.
Therefore, I entered the lab at the end of my freshman year. After several rotations, I decided to join the Host-Microbe Molecular Interaction Laboratory. The boss of the lab is professor Qin, a rigorous but gentle professor cared for each student he mentored. Our lab has a favorable academic atmosphere that everyone can make their achievements through assiduous work and good collaborations. While working in the lab, seniors are always helpful, and professor Qin would like to talk with us about our study or college life every day. I like the way the lab operates, people communicate, and the life moves on.
Later on, I got an opportunity to lead a team to perform our own research. This period of research taught me to be both a team leader and a team player. As our lab is doing research on pathogenesis of Botrytis cinerea, we often knockout some genes to see how it performs on plant leaves, then we complement those genes to retest its pathogenicity. But in fact, the result can be rather confused, for Botrytis cinerea is a kind of multinuclear fungus. So we decided to design an experiment to figure out how its nuclei distribute during mitosis. At first, we proposed a hypothesis based on the theses we referred to, that the bigger the cell is, the more the nuclei it will get during mitosis. To prove the hypothesis, we discussed with our tutor, and worked out a feasible plan. In our plan, we tried to insert two kinds of antibiotic marks into different nuclei within a specific spore of Botrytis cinerea, and use different kinds of resistant plates to select laboratory strains. Then we can subculture the strains to isolate monospore strains to do further research. I assigned tasks for everyone, hence we collaborated well to advance the process in an effective way. Everything got off the ground as I did excellent in proposal presentation and defense; we successfully designed and constructed two vectors, reorganized plasmids, transformed plasmids into agrobacterium, did preliminary experiment to determine the concentration of antibiotics. But after that, when it came to co-transformation, we really got into trouble. For a period of co-culture is about two weeks, and after two rounds of co-culture, we failed to obtain transformants. Then we analyzed the reason, did another co-culture. Again, we failed. We were puzzled and consulted our tutor for more advice. Under his instructions, my teammates and I then performed twenty-three rounds of co-culture, changing experiment conditions each time. We considered various pollutions, vitality of Botrytis cinerea, concentration of antibiotics, even changed G418 into chlorimuron-ethyl. But unfortunately, we failed all the experiments, sometimes we just got false positive transformants. That were definitely the gloomiest days we’d ever had during college life. I’d recall those days when I struggled to get out of my bed every morning, walked along the footpath to embrace the dark, bleak, winter crepuscule, only the Venus high hung, my footsteps echoed. Along the path stands a statue, with three rings around. I took that three rings as Hope, Courage and Life to me. Every time I passed it, strengths could gain from it to prop me up to be persistent. I was glad that my teammates could still be there to stand by me though they were also frustrated those days. The next summer vacation, I even stayed at the school.
My thirst for knowledge is strong since I was young. I used to listen to my parents telling stories, and became curious about the natural world, thirsting for exploring it. When grew up, I was no longer content with those stories, so I began my own reading. I subscribed to We Love Science since elementary school, read lots of discoveries, wondering my own adventures someday. That’s the first time I had an interest in science.Upon my entering Jilin University, I knew nothing about life science though. I chose to major in biotechnology just because I wanted to experience what I’ve read about in We Love Science when I was young. But at the end of my first semester, when I performed excellently in my academy and was given an opportunity to change my major, I hesitated. It was then I entered a class called Introduction to Life Science. During this general education course, the professor showed us the beauty and the charm of life science through his poetic description, passionate speech, vivid presentation. I was enchanted by this burgeoning field at once. When he explained to us how they finally managed to knockout some genes of the blue fox, changed its original white coat color, and decoded the cause of its death in ecstasy, I could feel his excitement, too, though I knew nothing about molecular genetics then. Through the course, I came to realize how wonderful life exists and brings about. At the end of this course, I made my decision to stay, for a keen fascination with life science. From then on, my journey was setting out to explore the colorful biology world.Therefore, I entered the lab at the end of my freshman year. After several rotations, I decided to join the Host-Microbe Molecular Interaction Laboratory. The boss of the lab is professor Qin, a rigorous but gentle professor cared for each student he mentored. Our lab has a favorable academic atmosphere that everyone can make their achievements through assiduous work and good collaborations. While working in the lab, seniors are always helpful, and professor Qin would like to talk with us about our study or college life every day. I like the way the lab operates, people communicate, and the life moves on.Later on, I got an opportunity to lead a team to perform our own research. This period of research taught me to be both a team leader and a team player. As our lab is doing research on pathogenesis of Botrytis cinerea, we often knockout some genes to see how it performs on plant leaves, then we complement those genes to retest its pathogenicity. But in fact, the result can be rather confused, for Botrytis cinerea is a kind of multinuclear fungus. So we decided to design an experiment to figure out how its nuclei distribute during mitosis. At first, we proposed a hypothesis based on the theses we referred to, that the bigger the cell is, the more the nuclei it will get during mitosis. To prove the hypothesis, we discussed with our tutor, and worked out a feasible plan. In our plan, we tried to insert two kinds of antibiotic marks into different nuclei within a specific spore of Botrytis cinerea, and use different kinds of resistant plates to select laboratory strains. Then we can subculture the strains to isolate monospore strains to do further research. I assigned tasks for everyone, hence we collaborated well to advance the process in an effective way. Everything got off the ground as I did excellent in proposal presentation and defense; we successfully designed and constructed two vectors, reorganized plasmids, transformed plasmids into agrobacterium, did preliminary experiment to determine the concentration of antibiotics. But after that, when it came to co-transformation, we really got into trouble. For a period of co-culture is about two weeks, and after two rounds of co-culture, we failed to obtain transformants. Then we analyzed the reason, did another co-culture. Again, we failed. We were puzzled and consulted our tutor for more advice. Under his instructions, my teammates and I then performed twenty-three rounds of co-culture, changing experiment conditions each time. We considered various pollutions, vitality of Botrytis cinerea, concentration of antibiotics, even changed G418 into chlorimuron-ethyl. But unfortunately, we failed all the experiments, sometimes we just got false positive transformants. That were definitely the gloomiest days we’d ever had during college life. I’d recall those days when I struggled to get out of my bed every morning, walked along the footpath to embrace the dark, bleak, winter crepuscule, only the Venus high hung, my footsteps echoed. Along the path stands a statue, with three rings around. I took that three rings as Hope, Courage and Life to me. Every time I passed it, strengths could gain from it to prop me up to be persistent. I was glad that my teammates could still be there to stand by me though they were also frustrated those days. The next summer vacation, I even stayed at the school.
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