安康地区金黄色葡萄球菌感染耐药性及相关基因检测张博1 徐纪茹2 周惠娟

安康地区金黄色葡萄球菌感染耐药性及相关基因检测
张博1 徐纪茹2 周惠娟 3 邱辉3
（1西安交通大学医学部，陕西西安 710061；2西安交通大学医学部病原生物教研室，陕西西安 710061 ；
3 安康中心医院检验科 陕西安康 725000）

摘要：目的 研究安康地区金黄色葡萄球菌（SA)的耐药表型及耐药基因携带情况，为指导临床抗感染治疗和控制院内感染提供参考依据。 方法 收集2014年5月-2015年3月临床分离标本54株金黄色葡萄球菌，采用K-B法和PCR法，检测其耐药性及相关耐药基因mecA、femA及qacA/B。 结果 金黄色葡萄球菌耐药率较高的抗菌药物为青霉素G、头孢呋辛、红霉素、克林霉素，耐药率分别为96.30%、85.29%、66.67%、62.96%，对万古霉素敏感度最高，其次为氯霉素、复方新诺明。54株SA中检出23株耐甲氧西林金黄色葡萄球菌（MRSA),检出率为42.59%（23/54）。54株SA中mecA、femA和qacA/B基因的阳性率分别是42.59%、98.15%、7.41%。而在金黄色葡萄球菌mecA基因阳性中femA和qacA/B基因的阳性率分别为100.00%、17.39%。结论 安康地区医院感染金黄色葡萄球菌对多重耐药的耐药率较高，未发现万古霉素耐药菌株，耐药基因mecA、qacA/B携带率较高。

結果 (中文) 3:

Drug resistance and related genes of Staphylococcus aureus infection in Ankang area detectionZhang Bo 1 Xu Jiru 2 Zhou Huijuan 3 Qiu Hui 3(1 Department of medicine at the Xi"an Jiao Tong University, Xi"an 710061, Shaanxi 2; Xi"an Jiao Tong University Medical Department of pathogenic biology department, Shaanxi Xi"an 710061;Department of clinical laboratory Ankang 3 Central Hospital of Shaanxi Ankang 725000)Abstract: Objective To study the drug resistance phenotype and drug resistance gene of Staphylococcus aureus (SA) in Ankang, and to provide reference for guiding clinical treatment and control of nosocomial infection. Methods 54 strains of Staphylococcus aureus were collected from March -2015, May 2014. K-B and mecA were used to detect the drug resistance and the resistance gene PCR, femA and qacA/B. Results in the high rate of drug resistance of Staphylococcus aureus antimicrobial agents for penicillin G, cefuroxime and erythromycin and clindamycin, resistance rate 96.30% and 85.29%, 66.67%, 62.96%, respectively, to vancomycin is most sensitive, followed by chloramphenicol, trimethoprim sulfamethoxazole. 23 strains of SA were detected in 54 strains of resistant Staphylococcus aureus (MRSA), the detection rate was 42.59% (23/54). The positive rate of SA in 54 strains of mecA, femA and qacA/B genes were 42.59%, 98.15%, 7.41%. While the positive rate of the mecA gene of Staphylococcus aureus in positive femA and qacA/B gene were 100%, 17.39%. Resistance to multiple drug resistance rate of Staphylococcus aureus infection results in hospitals of Ankang area, no vancomycin resistant strains were found. The higher positive rate of resistance genes mecA, qacA/B.