Washed platelets was prepared as described above and treated or
untreated with 5 μ
M prostaglandin E1 (PGE1) at 37 °C for 30 min [16],
followed by incubation with Sal-B (70, 140, 280
μ
M) or AR-C69931MX
(100 n M, a gift from Astra Zeneca, Loughborough, UK) under stirring
condition (900 rpm) for 3 min