After electrophoresis, the gel was

After electrophoresis, the gel was stained with ethidium bromide 1 lg mL )1 and washed for 10 min. Subsequently the gel image was acquired with a Gel Doc 2000 (Biorad). The amplified products (with a size of approximately 336 bp) were cloned, sequenced, and sequence comparison was performed. Hence, a reference ladder was made by adding an equal amount (about 200 ng of PCR fragments) of the rpoB amplicons and used in the DGGE analysis.

結果 (中文) 1:

后电泳，凝胶沾满了溴化乙锭溴 1 lg 毫升） 1 和水洗 10 分钟。随后凝胶图像被收购与凝胶 Doc 2000 (Biorad)。扩增的产物 (大小约 336 bp) 克隆、 测序，并进行了序列比较。因此，参考梯子由加上相等的金额 (约 200 PCR 片段吴) 的 rpob 基因扩增和 DGGE 分析中使用。

結果 (中文) 3:

电泳后的凝胶，用溴化乙锭染色）1毫升1 LG洗10分钟，随后凝胶图像用Gel Doc 2000收购（BioRad）。扩增产物（约336个基点）的扩增产物进行克隆，测序，并进行序列比较。因此，参考阶梯加等量制成（约200纳克的PCR片段的扩增和rpoB）用于DGGE分析。