CFU of C. perfringens were not enum

CFU of C. perfringens were not enumerated. Rather, this organism was monitored by presence or absence. SFP agar (Difco, Detroit, Michigan, USA) was sterilized then Bacto-egg yolk additive (Difco, Detroit, Michigan, USA), polymyxin B sulphate (Difco, Detroit, Michigan, USA) and kanamycin sulphate (Difco, Detroit,Michigan, USA) were mixed into the agar and the entire mixture was poured into screw cap test tubes. The agar was warmed to liquefy, prior to inoculation. Approximately 1 mL of agar was removed and replaced with 1 mL of well water and thoroughly mixed. No air space was left in the test tubes to create an anaerobic environment. Samples were incubated at 37 °C. The presence of the organism was indicated if black colonies were present. Tubes were checked for positive reaction after 24 hours and again after 1 week.
Samples that were positive after 24 hours were removed from further incubation and sterilized. Those that did not have any black colonies present after one week were considered to not contain C. perfringens. Two replicates of each test were performed.

結果 (中文) 1:

产气荚膜 CFU 不列举。相反，这种生物的存在或不存在进行了监测。SFP 琼脂 （肉汤，底特律，密歇根州，美国） 被消毒然后失业率是鸡蛋蛋黄添加剂 （肉汤，底特律，密歇根州，美国）、 多粘菌素 B 硫酸盐 （肉汤，底特律，密歇根州，美国） 和硫酸卡那霉素 （肉汤，底特律，密歇根州，美国） 喜忧参半进琼脂培养基和整个混合物被注入螺帽试管。琼脂被加热液化，在接种前。约 1 毫升的琼脂是删除和替换 1 毫升的井水和彻底混合。没有空气空间被留在试管创造厌氧环境。样品孵育在 37 ° c。有机体的存在表示是否黑色殖民地被提出。管为阳性反应后 24 小时和 1 个星期后再检查。24 小时后阳性的样品被删除从进一步孵化和消毒。没有任何黑色的殖民地后一周出现的那些被认为不包含荚膜。两个复制的每个测试被执行。

結果 (中文) 3:

产气荚膜梭菌没有列举数。相反，这种微生物的存在或不存在监控。SFP琼脂（Difco公司，底特律，密歇根，美国）消毒后细菌蛋黄添加剂（Difco公司，底特律，密歇根，美国），硫酸多粘菌素B（Difco公司，底特律，密歇根，美国）和硫酸卡那霉素（Difco公司，底特律，密歇根，美国）分别加入琼脂和整个混合物倒入螺丝帽试管。琼脂加热溶解，接种前。大约1毫升的琼脂被除去，并用1毫升的水和彻底混合取代。在试管中没有空气空间，创造一个厌氧环境。样品在37个C培养的有机体的存在下，表示如果黑色殖民地。1周后24小时后检查是否为阳性反应。24小时后阳性的样品被删除，从进一步培养和消毒。那些没有任何黑色的菌落一周后被认为不含产气荚膜梭菌。每个测试重复进行。