Target sample size was calculated to include 15 participants per group to detect minimum differences of 0.3 mmol/L in serum total cholesterol and 0.2 mmol/L in LDL cholesterol with 80% power based on our previous feasibility study (13). All statistical tests were 2 tailed, with significance level set at 0.05. SPSS for Windows (version 15.0; SPSS) was used for the statistical calculations.