While some products overexpressed in cells transformed by eIF4E have been identified, their individual and specific contribution to the malignant phenotype has not been elucidated. In an attempt to carry out a systematic identification of all the transcripts that depend on excess eIF4E/4F we have generated a subtraction cDNA library from the heavy polysomes of CHO cells overexpressing eIF4E (CHO-4E; Abid et al., 1999).