Treatment of Data : Determine the efficiency of the column of the column by calculating the number of theoretical plates N, using the retention time tR and the base peak width Wb: Prepare working curve for sample (plot the ratio of the peak area of the component relative to the peak area of the internal standard vs. concentration.) Determine the components in your unknown and the concentration of each from the standard curves. Identify the individual compounds in the sample from the UV spectrophotometry and a comparison with published retention data.