The intracellular and extracellular

The intracellular and extracellular PrA activities of mutant strains were measured under the same conditions. As shown in Fig. 2, the intracellular and extracellular PrA activity of the recombinant RY1-PEP4 was 0.43  105 U/mg and 0.31  105 U/mL,which decreased by 96% and 97%, respectively, compared with the parental strain RY-1. These results confirmed that PEP4 was
disrupted and deletion of the PEP4 could significantly decrease the PrA activity of S. cerevisiae.

結果 (中文) 1:

在相同条件下测定了突变株的细胞内和细胞外 PrA 活动。如图 2 所示，胞内和胞外 PrA 的重组的 RY1 PEP4 活性 0.43 10 5 U/mg 和 0.31 10 5 U/mL，分别下跌 96%和 97%，与父母应变比较 RY 1。这些结果证实 PEP4中断和删除 PEP4 能显著降低酵母的 PrA 活动。

結果 (中文) 3:

细胞内和细胞外的相同条件下测定PRA活性的突变株。如图2所示，其胞内和胞外的重组ry1-pep4 PRA活性0.43 105 U /毫克和0.31 105 U/ml，分别下降了96%和97%，分别与亲本菌株相比ry-1。这些结果证实了PEP4中断和PEP4缺失可显著降低PRA活性酵母。