Strawberries are a popular berry fruit consumed in the United States (8), although only a few studies examined previously their effects on CVD risk factors, including lipid profiles in adults. In a 3-wk randomized crossover trial, Zunino et al. (14) reported the effects of FDS (∼320 g of frozen strawberries) in decreasing plasma concentrations of total cholesterol, but they observed no effects on LDL and HDL cholesterol or on TGs in obese individuals with above-optimal LDL cholesterol concentrations. This study also controlled for background dietary intake by providing prepared meals and supervised meal visits for all participants for the study duration (14). In another 6-wk study, FDS (∼110 g of fresh strawberries) were shown to suppress postprandial increases in serum TGs in hyperlipidemic adults (16). Our 12-wk study shows the total and LDL cholesterol lowering effects of strawberries, which were observed only at high doses, in obese adults with above-optimal and/or borderline high LDL concentrations. Our findings, in concert with previous studies, provide evidence on the safety and efficacy of FDS, as a well-tolerated low-calorie dietary supplement in the nutritional management of elevated lipid profiles. Furthermore, berry anthocyanins in animal and cell models were shown to inhibit gastric lipase (34) and cholesteryl ester transfer protein (27) and decrease body fat deposits (35). These mechanisms may explain improved serum lipid profiles observed in clinical trials and warrant additional investigation, especially in context of caloric restriction and/or statin therapy.