A simple method based on laboratory

A simple method based on laboratory inoculum and field inoculum for evaluating
potato resistance to black scurf caused by Rhizoctonia solani
Xiao-Yu Zhang†
, Xiao-Xia Yu†
, Zhuo Yu*, Yu-Feng Xue and Li-Peng Qi
College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China
A two-step method was developed to evaluate potato resistance to black scurf caused by Rhizoctonia solani.
Tuber piece inoculum was first conducted in the laboratory, which was also first reported in this study. After
inoculation with pathogen discs and culture for 48 h, the necrotic spots on the inoculated potato pieces were
generated and measured by the crossing method. Further evaluation was conducted through field experiments
using a wheat bran inoculum method. The wheat bran inoculum was placed into the pit dispersedly and surrounded
seed tubers. Each cultivar or line was subjected to five treatments of 0-, 2-, 3-, 4-, and 5-g soil inoculum.
The results showed that 2–4 g of wheat bran inoculum was the optimum for identifying tuber black scurf
resistance. The laboratory scores positively correlated with the incidence and severity of black scurf in the
field. According to the results in the laboratory, relatively resistant cultivars could be selected for further estimation
of tuber black scurf resistance in field experiments. It is a practical and effective screening method for
rapid identification of resistant potato germplasm, which can reduce workload in the field, shorten time required
for identification.
Key Words: potato, Rhizoctonia solani, black scurf, wheat bran inoculum, tuber piece inoculum, two-step
method.

結果 (繁體中文) 1:

一種基於實驗室菌劑及其評價領域接種的簡單方法馬鈴薯抗性造成水稻紋枯病菌的黑色頭屑蕭昱 Zhang†曉霞 Yu†卓玉 *、 薛玉鋒、 李朋齊內蒙古農大農學院呼和浩特 010019，中國兩步方法來評估黑皮屑引起水稻紋枯病菌的馬鈴薯抗性。塊莖片接種進行第一次在實驗室裡，在這項研究還首次報導。後病原體光碟和 48 小時區域性的接種，接種的土豆塊壞死斑點了生成和過境法測定。通過田間試驗進行進一步的評價使用小麥麩皮接種方法。小麥麩皮接種被放進了坑裡分散和包圍種薯。每個品種或線遭受到五個治療的 0-2-3-，4-、 5-g 土壤接種。結果表明，2-4 g 的小麥麩皮接種鑒定塊莖黑皮屑最適電阻。實驗室成績呈正相關的發病率和嚴重程度在黑色頭屑欄位。根據實驗室的結果，可以為進一步估計選擇相對較抗病品種塊莖黑皮屑耐藥的田間試驗。它是一種實用、 有效的篩選方法抗性馬鈴薯種質資源，可以減少外地工作量，縮短所需時間的快速鑒定為標識。關鍵字： 馬鈴薯、 水稻紋枯病菌、 黑皮屑、 小麥麩皮接種、 塊莖片接種、 兩步法方法。