Thus, the user once receiving a container such as that shown in FIG. 4 with a sample contained therein, various tests can be performed on a sample. Covering a substantial portion of the length of the tube is a multi-layer barrier coating . Multi-layer barrier coating covers substantially most of the tube with the exception of open end thereof. Multi-layer barrier coating comprises a first layer of a polymer material such as an acrylate, a second layer of mixture of a metal oxide such as SnOx, GeOx,or PbOx, and a silicon oxide material and a third layer of an organic barrier material such a PVDC.