Today’s “high fidelity” DNA polymerases feature even more detailed engineering. For example, the Thermo Scientific Phusion High-Fidelity DNA Polymerase is an archaebacterial enzyme with proofreading function alongside a thermostabile DNA binding protein. “This binding protein increases affinity of the polymerase for double-stranded DNA,” says Smergeliene. “The high processivity and robustness of Phusion make the enzyme extremely resistant to PCR inhibitors and enable additional applications not feasible with other more standard polymerases.” EMD Millipore’s high fidelity KOD DNA polymerase is also designed for new PCR applications, such as next-generation sequencing (NGS), developing synthetic biology constructs. “Our PureGenome™ Next Generation Sequencing kits include the benefits of KOD DNA Polymerase to reduce NGS library preparation bias, a common problem in high-throughput PCR-based sequencing strategies,” says Ayaz Majid, product manager for genomics at EMD Millipore.