Trypophobia, or the fear of small clusters of objects in close proximity to one another is a unique and recently discovered phenomenon to the scientific literature. Recent work has shown that a small subset of the human population will react adversely to trypophobic images. Although trypophobia at first glance seems irrational, these images might be triggering a primitive threat detection system that has adaptive functionality - trypophobic images share similar spectral characteristics also found among dangerous animals. In the present study, we replicate previous work showing increased levels of discomfort when viewing trypophobic images and also provide further validation for a newly designed questionnaire that measures participants level of trypophobia. Measuring participant physiology, we then show an increase in two measures of electrodermal activity when participants viewed trypophobic images compared to control images. Our results add to the accumulating literature investigating the adaptive rationale for exhibiting heightened fear responses when viewing trypophobic imagery.