Seventy-seven LAB isolates obtained

Seventy-seven LAB isolates obtained from naturally fermented pastes prepared from mackerel
(Scomber scombrus Linnaeus 1785) and sucrose (22:3, w/w) incubated at 15 and 228C were screened for their ability to produce biogenic amines by decarboxylation of the corresponding amino acids (histidine, tyrosine, lysine and ornithine). Histidine,tyrosine, lysine and ornithine degradation were tested in a pH-based differential medium for the detection of biogenic amine formation, the modified Moeller broth (Joosten and Northolt, 1987). Histamine for mation was confirmed by qualitative thin-layer chro matography, according to Lieber and Taylor (1978),using silica-gel plates (F , 20 254 3 20 cm, thickness of layer: 0.25 mm; Merck no. 5715, Darmstadt, Germany). Aliquots of 10 ml of modified Moeller broth inoculated with the bacterial cultures under study and incubated at 308C for 48 h were spotted onto the plates. Samples of modified Moeller broth containing added histamine were also spotted as a control. The samples were eluted with methanol–ammonia (20:1). After drying, the plates were de
veloped with ninhydrine spray reagent (300 mg of ninhydrine, 100 ml of n-butanol, 3 ml of glacial acetic acid). Histamine was detected as violet-grey spots (Rf 5 0.74). The detection level for this method 21 was 0.01 g l .

結果 (中文) 1:

七十七个实验室分离物自然发酵浆料制备鲭鱼(调制 scombrus 林奈 1785年) 和蔗糖 (22:3，w/w) 在 15 和 228 C 孕育产生对他们能够生产生物胺进行筛查通过脱羧反应的相应的氨基酸 (组氨酸、 酪氨酸、 赖氨酸、 鸟氨酸)。组氨酸、 酪氨酸、 赖氨酸、 鸟氨酸降解的生物胺形成，改性的默肉汤 (Joosten 和诺索尔特，1987年) 检测 ph 值基于微分介质中进行了测试。组胺变换证实了定性的薄层色谱定性分析，根据尼博和泰勒 (1978 年)，使用硅胶板 (F，20 254 3 20 厘米，层厚度: 0.25 m m;默克号 5715，达姆施塔特，德国)。10 毫升的改性默肉汤接种下研究的细菌培养和孵化在 48 h 308 C 整除的数，并发现盘子。样品的改性默肉汤含有添加组胺，有人还看见作为控制。样品用甲醇-氨水 (20:1) 洗脱。干燥后，盘子被德与 ninhydrine 喷雾试剂 (300 毫克的 ninhydrine，100 毫升的丁醇-冰醋酸 3 毫升) 的研制。组胺被检测为紫灰色斑点 (射频 5 0.74)。此方法 21 的检测级别是 0.01 g l。

結果 (中文) 3:

七零七实验室分离制备得到的自然发酵糊鲐（鲐鲅鱼Linnaeus 1785）和蔗糖（22:3，w/w）在15和228c培养筛选其由相应的氨基酸脱羧产生生物胺的能力（组氨酸、酪氨酸、赖氨酸、鸟氨酸）。组氨酸、酪氨酸、赖氨酸、鸟氨酸降解在pH值为基础的微分中生物胺形成的检测试验，改良默勒液（1987 Joosten和站，）。组胺形成的薄层色谱证实，根据利伯和泰勒（1978），用硅胶板（F 20 254 3 20厘米，土层的厚度：0.25毫米；默克5715号，达姆施塔特，德国）。等分的ML 10改性默勒肉汤接种在研究细菌在48小时后被发现在板308C。添加组胺的改性默勒肉汤样品也被发现作为一个控制。样品用甲醇洗脱–氨（20:1）。干燥后，板被实验采用茚三酮显色剂（300毫克的茚三酮，100毫升正丁醇、冰醋酸3毫升）。组胺检测为紫灰色点（射频0.74 5）。此方法的检测水平为21，为0.01克。