The cell line’s growth characteristics are another consideration, notes Mark Rothenberg, applications manager at Corning, because the required vessel volume depends on the number of cells you require, the cells’ doubling times and the degree of proximity the cells require for ideal growth. “A culture plan should be developed for scaling up the cells, taking into account the [cells’] characteristics and time line,” Rothenberg says. “Utilizing vessels that range in size from T25cm2 to T225cm2 will get to the goal, but there are newer alternatives that may be more beneficial. These include multilayer flasks or HYPERFlasks® from Corning®.” Sigma-Aldrich is also in the process of launching a line of cultureware, including tissue-culture treated flasks, serological pipettes, conical tubes and cryovials.