Of the prepared dispersions, 5 ml was dropped into the specific centrifuge tubes. The centrifuging was performed at 15 minutes in 1000 and 4000 rpm. Afterward, 0.1 ml of each dispersion is dropped into a gouge balloon and diluted with 5 ml of acetone. The concentrations of prepared solutions in the water were determined by a ultravioletvisible (UV-vis) spectrophotometer at the dye’s wavelength of maximum absorption (498nm).