Cells were transfected with a 20-nt

Cells were transfected with a 20-nt guide directed toward ACTC1, 14-nt guides directed toward MIAT and TTN and either Cas9-VPR or Cas9. This picture represents the expected behavior of Cas9-VPR. The ACTC1 locus should be cut, while transcription occurs for the
genes MIAT and ACTC1.

結果 (中文) 1:

针对 ACTC1，14-nt 指南针对利宾和 μ 和 Cas9 VPR 或 Cas9 20-nt 指南转染细胞。这幅画描绘 Cas9 VPR 的预期的行为。ACTC1 轨迹应该削减，而转录发生利宾和 ACTC1 的基因。

結果 (中文) 3:

细胞转染20 nt的引导指向ACTC1，14 NT引导朝向MIAT和TTN和Cas9 VPR或Cas9。这幅画描绘了Cas9 VPR的预期行为。的ACTC1轨迹应该削减，而转录发生的
MIAT和ACTC1基因。