The whisker pad was dissected to obtain isolated vibrissae follicles with forceps and fine needles, from mice anesthetized using a ketamine mixture (intramuscular injection of a 0.02 ml solution of 20 mg/kg ketamine, 15.2 mg/kg xylazine, and 0.48 mg/kg acepromazine maleate), using a binocular microscope (MZ6, Leica, Weztlar, Germany). The isolated vibrissae hair follicles with their capsules were used for Gelfoam histoculture.
The whisker pad was dissected to obtain isolated vibrissae follicles with forceps and fine needles, from mice anesthetized using a ketamine mixture (intramuscular injection of a 0.02 ml solution of 20 mg/kg ketamine, 15.2 mg/kg xylazine, and 0.48 mg/kg acepromazine maleate), using a binocular microscope (MZ6, Leica, Weztlar, Germany). The isolated vibrissae hair follicles with their capsules were used for Gelfoam histoculture.
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