Here, we show that ENO1 is widely expressed in normal human tissues and is significantly upregulated in most TCGA (The Cancer Genome Atlas) cancer patients. Furthermore, OS analysis found that high expression of ENO1 was significantly associated with poor prognosis of BLCA, BRCA, CESC, ESCC, HNSC, LIHC, LUAD,PAAD, and SARC cancers. Concerning the RFS, high expression of ENO1 was significantly related to poor prognosis of BRCA, LIHC,SARC, PAAD, and TGCT cancers.