The HPLC analysis showed a concentration of 9.5% of anthocyanins in the CAE. The initial anthocyanin and rutin concentrations in CAE and R juices was of 1.3 and 10.1 mg/100 mL respectively (Fig. 3). These amounts of anthocyanins and rutin were lower than the expected (1.9 and 15 mg/100 mL respectively) due to the lower solubility of anthocyanins and rutin in aqueous media respect to acidified methanol.