A more practically-oriented segmentation method for detecting/segmenting abnormal nuclei within a field-of-view (FOV) had recently been reported and evaluated by our group . Specifically, a local adaptive graph cut (LAGC) approach, which models the nucleus and background as two Poisson distributions is proposed to refine the coarse segmentation of both normal and abnormal nuclei. Recently, a deep learning initialization and super pixels graph cut refinement method was proposed by our group with the aim of improving the segmentation of nuclei . Our previous approaches work well in most situations although they may generate inaccurate boundaries when the nuclei exhibit poor staining and/or their boundary contrast is low.In order to handle the challenges