Trans-10,cis-12 CLA was infused into the abomasum using a 0.5-cm i.d. line placed through the rumen cannula. An initial priming dose of 6.25 g of CLA was given at time zero, followed by 2.1 g every 4 h for 36 h and then 3.1 g every 6 h until 120 h. This provided 12.5 g/d of trans-10,cis-12 CLA, which is slightly over the maximally effective dose (~10 g/d; de Veth et al., 2004).