摘要： 目的 通过实验初步掌握人类染色体的分带技术，了解人类染色体的G

摘要： 目的 通过实验初步掌握人类染色体的分带技术，了解人类染色体的G带的分析方法以及姐妹染色单体色差分析的原理和方法，通过姐妹染色单体互换（sister chromatid exchange, SCE）标本的观察，掌握SCE的计数方法。方法 在人类染色体遗传病研究中，我们可以通过胰酶消化、Giemsa染液染色等方法对染色体标本进行显带处理。本实验主要通过染色体G带技术对标本进行了显带。同时，本实验还通过BrdU-Giemsa法，对染色体标本进行了紫外灯照射，配合2×SSC溶液作用和Giemsa染液处理，我们对染色体标本进行了SCE观察计数。结果 姐妹染色单体色差分析实验结果显示，部分染色体标本出现了明显的色差现象以及染色单体互换，但染色单体互换频率普遍不高30个人外周血淋巴细胞姐妹染色单体互换的平均值为1.57。
关键词： 染色体；人体外周血淋巴细胞；G带染色技术；姐妹染色单体区分染色法

結果 (英文) 1:

Summary: aim through experiments with human chromosome banding techniques, understanding of human chromosome g-banding analysis method and analysis of sister chromatid color principles and methods, through sister chromatid exchanges (sister chromatid exchange, SCE) specimen observation, master SCE counting methods. Methods in human chromosomes in the study of genetic diseases, we can by trypsin digestion, Giemsa dye dyeing methods of chromosome banding. This study by chromosome g-banding technique for specimens. Meanwhile, the experiment through the BrdU-Giemsa method, the chromosome is UV light irradiation, with the 2xSSC solution and Giemsa staining solution, our SCE observation of chromosome counts. Resulting sister chromatids colour difference results display, visible chromatic aberration phenomenon in a part of the chromosome and chromatid, chromatid exchange frequency is not high at 30 of sister chromatid exchange in human peripheral blood lymphocytes average was 1.57.Key words: chromosome; human peripheral blood lymphocytes; g-banding staining techniques; sister chromatids distinguish staining

結果 (英文) 2:

Abstract: Objective To master the experimental human chromosome banding techniques, understanding of the principles and methods of analysis and sister chromatid color G with human chromosomes analyzed by sister chromatid exchange (sister chromatid exchange, SCE) specimens observation, grasp SCE counting method. Methods of human genetic disease chromosomes, we can trypsin digestion, Giemsa dye staining method for chromosome banding process samples. This experiment by chromosome G-banding technique specimens were banding. Meanwhile, the experiment also by BrdU-Giemsa method, chromosome specimens were irradiated with UV light, with 2 × SSC solution and Giemsa dye effect treatment, we observed SCE chromosome specimens were counted. Results Sister chromatid color analysis of experimental results show that a significant portion of chromosome specimen of the phenomenon of color and chromatid exchanges, but generally not high chromatid exchange frequency in peripheral blood lymphocytes of 30 people of sister chromatid exchange an average of 1.57.
Keywords: chromosome; human peripheral blood lymphocytes; G band staining techniques; sister chromatid distinguish staining

結果 (英文) 3:

Abstract: Objective To investigate the initial grasp of human chromosome banding technique, to understand and analyze the method of human chromosome G banding and sister dyeing principle and the method of analysis of single color, excliange by staining (sister chromatid exchange, and SCE) were observed, counting method SCE. Methods in the study of human genetic diseases, we can on chromosome banding were treated by trypsin digestion and Giemsa dyeing method. This experiment mainly through chromosome G band technology on the specimens were banding. At the same time, the experiment also by BrdU-Giemsa method, on the chromosome were studied by ultraviolet irradiation, with 2 x SSC solution and Giemsa staining, we observed by SCE on chromosome counting. The experimental results of single color sister chromosome analysis showed that the chromosome aberration in specimen appeared obvious and chromatid exchange, but chromatid exchange is generally not high 30 peripheral blood lymphocyte sister chromatid exchange staining average value is 1.57.Key words: chromosome; human peripheral blood lymphocytes; G band staining; sister chromatid differential staining