In the denitrification process, heterotrophic bacteria use nitrate as<br>an electron acceptor in the absence of oxygen, and organic matter as a<br>carbon source, resulting in the reduction of nitrate to gaseous nitrogen<br>N2, which is released directly into the atmosphere, as can be observed<br>by model proposed by Wiesmann (1994), using acetate as carbon<br>source. The application of denitrification in the biofloc system would<br>contribute to the control of nitrate accumulation and reduce the soluble<br>organic matter inside the tank (Schreier and Zohar, 2006). The removal<br>of nitrogen forms from the system allows the treated water to be reused<br>without the need for renewal. Additionally, treated water with low<br>suspended solids content can be sterilized by simplified physicochemical processes such as ultraviolet radiation, foam fractionators, ozone to<br>eliminate potential pathogen contamination and reused directly in the<br>production cycle, promoting healthy shrimp growth.
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