OEG suspensions were prepared as Ru

OEG suspensions were prepared as Ruitenberg et al. described. Purified OEGs were seeded onto poly-L-lysine-coated dishes at a density of 1×106 cells in DF-10S. The next day, the medium was replaced with fresh medium containing pcDNA3.1(+)-NT3 or pcDNA3.1(+), and cells were left for 72 h. After that, the OEG cultures were washed with L-15 medium, detached by trypsinization and washed twice in serum-free DMEM/F-12 medium. Next, cells were pelleted bylow-speed centrifugation,carefully resuspended, and diluted in appropriate volume of DMEM/F-12 to obtain the OEG suspension at 50 000 /μL. The viability of OEG suspensions, which was assessed by counting the percentage of dead cells using Trypan blue staining, was >95%.

結果 (中文) 1:

OEG 悬浮液准备 Ruitenberg et al.所述。纯化的 OEGs 接种到聚-L-赖氨酸-镀膜的菜肴，密度为 1 × 106 细胞的 DF-10S。第二天，介质被替换用含 pcDNA3.1 () 的新鲜培养基-NT3 或 pcDNA3.1 () 和单元格留给 72 h。之后，OEG 文化是用清水洗净 L-15 中等由 trypsinization 分离和洗过两次在无血清 DMEM/F-12 中等。下一步，单元格是颗粒的保护膜速度离心，仔细再悬浮，并在适当的卷的 DMEM/F-12 获得 OEG 悬浮在 50 000 /μL 稀释。OEG 悬浮液，生存能力是进行评估通过计数使用台盼蓝染色的死细胞的百分比，是 > 95%。

結果 (中文) 2:

OEG悬浮液制备为Ruitenberg等。描述。纯化OEGs接种到聚-L-赖氨酸包被的培养皿以1×106个细胞在DF-10S的密度。第二天，将培养基更换为新鲜的含介质的pcDNA3.1（+）-NT3或染pcDNA3.1（+），并且细胞被放置72小时。在此之后，OEG培养液用L-15培养基中，通过胰蛋白酶消化分离，并在无血清的DMEM/F-12培养基洗涤两次。接着，细胞沉淀bylow高速离心，小心悬浮和稀释DMEM/F-12适当的音量，以获得OEG悬挂在50 000 /μL。OEG混悬剂的可行性，这是通过计数死细胞用台盼蓝染色的百分比进行评估，为> 95％。

結果 (中文) 3:

制备的卢腾伯格等人OEG悬浮液。描述。纯化的嗅鞘细胞接种到聚-L-赖氨酸包被的菜肴的密度为1×106细胞在df-10s。第二天，培养基含有pcDNA3.1新鲜培养基替换（）和pcDNA3.1（-），和神经细胞72 h后，左，OEG培养物的L-15培养基洗涤，用胰酶消化法分离和洗涤两次在无血清的DMEM／F-12培养基。其次，细胞颗粒经济高速离心，仔细的再悬浮和稀释，在DMEM F-12适当体积的50／000／μL. OEG悬浮液的可行性得到OEG悬挂，这是由计数用台盼蓝染色死细胞百分比评估，＞95%。