1 mL of appropriate dilutions was taken to petri dishes and approx. 15-20 mL of Rogosa Agar were added until forming a thin layer and mixed.<br><br>After gelation of the media, petri dishes were incubated upside down at 37 oC for 3 days in aerobic conditions. <br><br>Following the incubation, round shaped yellowish colonies were counted and determined as B. longum count in CFU/g<br>(Lapierre, Undeland & Cox, 1992; Vinderola & Reinheimer, 1999).
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