Interfering substances. Interferenc

Interfering substances. Interference in the Fungitell assay due to hemolysis,
bilirubin, and lipemia was assessed by supplementation of a BG-positive serum
sample with each of the individual interferents (25). To prepare sufficient quantities
of BG-positive serum samples for this study, a unit of expired fresh frozen
plasma that tested negative for BG was clotted with CaCl2. Serum was collected
by centrifugation and sterilely filtered. A BG-positive serum sample control was
produced by reconstituting lyophilized BG from Associates of Cape Cod, Inc.,
with the above-processed serum to a concentration of 161 pg/ml. The BG standard
(pachyman) used in this study was the same as that supplied with the
Fungitell kit. Bilirubin and triglycerides (Intralipid) were purchased from Sigma,
St. Louis, Mo. A solution of hemoglobulin was prepared by lysis of washed red
blood cells with pyrogen-free water (Associates of Cape Cod, Inc.). Stock solutions
of bilirubin, triglycerides, and hemoglobulin, prepared in pyrogen-free
water, were added individually to aliquots of the BG-positive serum prepared
above to simulate a range of bilirubin, triglyceride, and hemoglobin concentrations
of approximately 0 to 200 mg/dl, 0 to 1,000 mg/dl, and 0 to 2,500 mg/dl,
respectively. Final concentrations of hemoglobin, bilirubin, and triglycerides
were determined for each aliquot. Bilirubin and triglycerides were determined
with a Modular Analytics P instrument (Roche Diagnostics Corporation, Indianapolis,
Ind.) using reagents supplied by the manufacturer. Hemoglobin concentration
was determined with a Beckman Coulter DU 800 spectrometer. Samples
supplemented with interferents were tested by the Fungitell assay and compared
to the BG-positive sample without an interferent. To estimate minimum interfering
concentrations, interference plots for hemoglobin, bilirubin, and triglycerides

結果 (中文) 1:

Interfering substances. Interference in the Fungitell assay due to hemolysis,bilirubin, and lipemia was assessed by supplementation of a BG-positive serumsample with each of the individual interferents (25). To prepare sufficient quantitiesof BG-positive serum samples for this study, a unit of expired fresh frozenplasma that tested negative for BG was clotted with CaCl2. Serum was collectedby centrifugation and sterilely filtered. A BG-positive serum sample control wasproduced by reconstituting lyophilized BG from Associates of Cape Cod, Inc.,with the above-processed serum to a concentration of 161 pg/ml. The BG standard(pachyman) used in this study was the same as that supplied with theFungitell kit. Bilirubin and triglycerides (Intralipid) were purchased from Sigma,St. Louis, Mo. A solution of hemoglobulin was prepared by lysis of washed redblood cells with pyrogen-free water (Associates of Cape Cod, Inc.). Stock solutionsof bilirubin, triglycerides, and hemoglobulin, prepared in pyrogen-freewater, were added individually to aliquots of the BG-positive serum preparedabove to simulate a range of bilirubin, triglyceride, and hemoglobin concentrationsof approximately 0 to 200 mg/dl, 0 to 1,000 mg/dl, and 0 to 2,500 mg/dl,respectively. Final concentrations of hemoglobin, bilirubin, and triglycerideswere determined for each aliquot. Bilirubin and triglycerides were determinedwith a Modular Analytics P instrument (Roche Diagnostics Corporation, Indianapolis,印第安纳州）使用由制造商提供的试剂。血红蛋白浓度用贝克曼库尔特杜 800 光谱仪测定了。样品辅以烟幕进行测试 Fungitell 测定和比较对 BG 阳性样品，不用组份。估计最小干扰浓度，干涉图为血红蛋白、 胆红素、 及甘油三酯

結果 (中文) 3:

干扰物质。由于溶血的干扰，测定胆红素和血脂fungitell
，由BG阳性血清
样品各干扰物补充评估（25）。准备足够数量
BG阳性的血清样本，在这项研究中，一个单位的新鲜冰冻血浆过期
检测阴性的BG是凝结与CaCl2。收集血清
通过离心和过滤除菌。BG阳性血清样品对照
重组冻干BG从科德角，公司员工生产，与上述处理
血清浓度为161 pg/ml，BG标准
（茯苓多糖）用于本研究，提供
fungitell盒一样。胆红素和甘油三酯（脂肪乳剂）均购自Sigma，
St.路易斯，用无热原的水洗涤红血细胞裂解制备Mo
溶液血红蛋白（科德角，公司员工）。
股票解胆红素，甘油三酯，和血红蛋白，在无热原
水配制，分别加入阳性血清制备的BG
以上模拟一系列的胆红素，甘油三酯等分，和血红蛋白浓度
大约0到200毫克/升，0到1000毫克/升，和0至2500毫克/分升，
分别。血红蛋白，胆红素的最终浓度，和为每个等分试样测定甘油三酯
。胆红素和甘油三酯测定采用模块化分析仪器
P（罗氏诊断公司，印第安纳波利斯，印第安纳
使用制造商提供的试剂）。
血红蛋白浓度与贝克曼库尔特杜800光谱仪测定。样品添加干扰物
由fungitell法测试和比较
对BG阳性样品无干扰。估计最小干扰
浓度，干扰图血红蛋白，胆红素，和甘油三酯