For all of these applications, the most general substrate sequence scope is desired, either from an individual DNA enzyme or collectively from a set of DNA enzymes. Such generality has been achieved
with RNA-cleaving deoxyribozymes49−51 and RNA ligase deoxyribozymes and seems likely for DNA-hydrolyzing
deoxyribozymes as well,45,54 suggesting the viability of general DNA-catalyzed oligonucleotide 3′-phosphorylation.