3.2. Determination of oligosacchari

3.2. Determination of oligosaccharide profile by LC–MS
The desalted enzymatic hydrolysate was then analysed by LC–MS. The total ion current chromatogram and extracted ion current chromatogram of LC–MS are shown in Fig. 1. The deduced composition of oligosaccharides in the enzymatic hydrolysate and their calculated distribution coefficients are listed in Table 1. Eight oligosaccharides were detected by LC–MS. There was only a single oligosaccharide for each degree of polymerisation, and the ion current intensities of trisaccharide and tetrasaccharide were significantly higher than the others. Compared with the complex pattern following mild acid hydrolysis of A. Molpadioides SC-FUC
(data not shown), the oligosaccharide profile of the enzymatic hydrolysate was much simpler. Moreover, distribution coefficients of the enzymatically prepared oligosaccharides were significantly different from each other, suggesting that those oligosaccharides could be effectively separated employing SEC. These evidences all reflected the advantage of enzymatic preparation.

結果 (中文) 1:

3.2.低聚糖形由液相色谱 — — 质谱法测定脱盐水的酶法水解液然后用液相色谱 — — 质谱分析。总离子电流色谱和液相色谱 — — 质谱提取的离子电流色谱图 1 所示。低聚糖在酶法水解液和其计算的分布系数的推导出的组成在表 1 中列出。采用液相色谱 — — 质谱检测八低聚糖。只有单一低聚糖对聚合，每个度，离子电流强度的四糖和三糖被有力地比别人高。相比后温和酸水解的参 SC FUC A.的复杂模式（未显示的数据），酶法水解低聚糖形要简单得多。此外，酶解制备的低聚糖的分布系数被有力地不同于彼此，暗示那些低聚糖能雇用秒有效分离所有这些证据反映酶制剂的优势。

結果 (中文) 3:

3.2。LC–MS测定寡糖亲fi乐
脱盐后的酶解液进行–LC MS总离子流图和提取离子流色谱LC–MS分析如图1所示。推导的成分低聚糖水解及其分布系数的计算fi系数列于表1。LC–女士有检出八只各寡糖聚合度单寡糖，和三糖、四糖的离子流强度明显高于其他fi。海地瓜SC-FUC后
温和酸水解的复杂模式相比（数据未显示），寡糖的亲fi乐酶解产物的简单多了。此外，分配系数fi所有酶的制备低聚糖的意义fi彼此明显不同，这表明这些低聚糖可有效地分离采用秒。这些证据都反映酶制剂的优势。