Variance components can be estimated by the method of moments from ANOVA Type III MS,
but two complications arise in the case of unbalanced data: First, such estimates are reasonable estimates if the data are not too badly balanced, but the statistical properties of such estimators are unknown, so it can be difficult to know how reliable they are for a given data set. Second, the expected mean squares shown for the balanced data set above are not correct for the unbalanced data case, as the coefficients on the variance components are affected by the data structure. The computation of the coefficients can be horribly complex (Milliken and Johnson, 2004; Rawlings et al., 2001), but we can get the coefficients using SAS Proc GLM with the random statement, resulting in this output: