However, bed-rest studies do not reflect typical human behaviour and experimental studies are now starting to focus on the impact of prolonged sitting. Just one day of prolonged sitting results in a significant reduction in postprandial glucose and insulin [47]. Recently, Dunstan et al [48] demonstrated that breaking up periods of prolonged sitting with 2-min bouts of light-intensity activity every 20 min in overweight and obese adults results in a 24% reduction in postprandial glucose AUC and a 23% reduction in insulin AUC, compared with uninterrupted sitting.