The discovery of catalytic RNAs (ri

The discovery of catalytic RNAs (ribozymes) sparked scientific interest directed to the preparation of new biocatalysts.1,2 Analogous deoxyribozymes (catalytic DNAzymes) are not available in nature, but have been demonstrated synthetically.3,4 An interesting DNAzyme that revealed peroxidase-like activities is a complex between hemin and a single-stranded guanine-rich nucleic acid (aptamer).5 This complex catalyzed the oxidation of 2,2¢-azino-bis(3-ethylbenzthi-azoline)-6-sulfonic acid, ABTS, by H2O2. It was suggested that the intercalation of hemin into the complex results in the formation of the biocatalyst. We have shown that the hemin/G-quadruplex also mimics peroxidase by the generation of chemiluminescence in the presence of H2O2 and luminol.7 The use of DNAzymes as catalytic labels for biosensing is attractive since nonspecific adsorption processes associated with protein-based labels are eliminated.

結果 (中文) 1:

发现的催化 Rna （核酶） 引发科学兴趣定向到新的 biocatalysts.1,2 类似脱氧核酶 （催化脱氧核酶） 的制备是不具备的性质，但已被证明 synthetically.3,4 有趣的脱氧核酶，揭示过氧化物酶样活性是氯化血红素和单链富含鸟嘌呤的核酸 （适）.5 这复杂之间的复杂催化氧化的 2，2、-联-bis(3-ethylbenzthi-azoline)-6-磺酸ABTS，用 H2O2。有人建议氯高铁血红素插层复合结果形成的生物催化剂。我们已经表明氯化血红素/G-四链体也模仿过氧化物酶化学发光法在 H2O2 代和 luminol.7 脱氧核酶作为催化标签用于生物传感是有吸引力因为消除非特异性吸附过程与基于蛋白质的标签相关联。

結果 (中文) 3:

催化RNA的发现（核酶）引发了科学的兴趣引导到新的生物催化剂的制备。1，类似的脱氧核酶（催化脱氧核酶）所不具备的性质，但已被证明是一个有趣的综合。3脱氧核酶显示过氧化物酶的活动是血红素和单链鸟嘌呤丰富的核酸之间的复杂（适体）。5这个复杂的催化氧化¢2,2 -双（3-乙基-苯并噻唑啉3-ethylbenzthi-azoline）- 6-sulfonic酸、ABTS、H2O2。建议在生物催化血红素形成的复杂结果插层。我们已经表明，血红素/体还模仿过氧化物酶的过氧化氢和鲁米诺化学发光产生的存在。7利用脱氧核酶催化的标签是有吸引力的以蛋白质为基础的生物传感自相关过程消除非特异性吸附标签。