Comparing the results of ESI–CID–MS

Comparing the results of ESI–CID–MS/MS and NMR, all the deduced information from ESI–CID–MS/MS was validated by NMR. It showed that MS is an efficient and sample saving procedure for
structural investigation of SC-FUC oligosaccharides and could facilitate the resolution of NMR spectra, although complete clarification of fine structure required more proof from NMR. Combining the investigation of NMR and MS/MS, the fine structures of four major oligosaccharides in the enzymatic hydrolysate of SC-FUC were clarified and are shown in Fig. 4B.It was found that the structures of those four oligosaccharides are very similar. Fraction II could be composed of fraction I and a nonsuphated fucose residue. Fraction III could consist of fraction II and fraction I. Fraction IV could be composed of two moieties of fraction II. These results suggest that disulphated trisfuose and disulphated tetrafucose might constitute a large proportion of the structural backbone of A. Molpadioides SC-FUC.
Meanwhile, other fucoidan oligosaccharides obtained from sea cucumbers and algae have been reported. Chen et al. (2012) obtained [3-a-L-Fucp(2,4OSO23)-1 ? 3-a-L-Fucp-(2OSO23)1 ? 3-a-L-
Fucp-(2OSO23)-a-L-Fucp-1 ? ]n(n = 1,2) from sea cucumber Isostichopus badionotus by mild acid hydrolysis. In comparison with these oligosaccharides, 2-O-sulphation was absent in our oligosaccharides, which indicates that the sulphated pattern of fucoidans might be distinct in different species of sea cucumber. Many researchers have suggested that fucoidan oligosaccharides obtained from algae were heterogeneous with various sugar compositions, O-acetylated groups, glycosidic bonds and branching (Bakunina et al., 2002; Bilan et al., 2005; Clement et al., 2010; Ye et al., 2005). Compared with these algae-derived oligosaccharides, echinoderm-derived oligosaccharides were more simple and regular

結果 (中文) 1:

ESI — — CID — — MS 和 NMR 结果比较，推导出了的索取 ESI — — CID — — MS 核磁共振法进行了验证。它表明 MS 是规避和样品保存程序SC FUC 低聚糖的结构调查并有助于核磁共振谱图的分辨率虽然完成 clarification 的合身结构需要更多的证据，从核磁共振。核磁共振和质谱研究相结合，SC FUC 酶水解液中的四个主要寡糖的合身结构，clarified 和图 4B 所示。它被发现的那些四的寡糖链结构都非常相似。组分 ⅱ 能组成分数第一和 nonsuphated 岩藻糖残渣。分数 III 可包括 II 和分数一、 分数第四可以组成两种基团的组分 ⅱ 的分数。这些结果表明 disulphated trisfuose 和 disulphated tetrafucose 可能构成结构骨干参 SC FUC A.的很大一部分。与此同时，有报道从海参和藻类获得其他褐藻糖胶寡糖。Chen 等人 （2012 年） 获得 [3 a L Fucp(2,4OSO23) 1 吗？ 3--L-Fucp-（2OSO23） 1? 3--L-Fucp-(2OSO23)-a-L-Fucp-1 吗？] n(n = 1,2) 从 Isostichopus badionotus 温和酸水解的海参。与这些低聚糖，2-O-硫酸盐化缺席在我们低聚糖，它指示岩藻聚糖硫酸化模式可能是不同的不同种类的海参。许多研究者已经表明获得从藻类的褐藻糖胶寡糖异构与不同的糖成分、 O-乙酰化团体、 糖苷键及分支 （Bakunina 等人，2002 年 ；比兰等人，2005 年 ；克莱门特 et al.，2010） ；叶等人，2005 年）。相比这些藻类寡糖，棘皮动物寡糖是更简单和定期

結果 (中文) 3:

比较–CID–ESI MS / MS和NMR的结果，所有推导的信息–CID–ESI MS / MS进行了核磁共振。结果表明，MS是一种高效、节能的EFfi样本程序的结构分析和
SC-FUC低聚糖可以促进核磁共振光谱的分辨率，虽然fiNE向构造完整的阐述fi阳离子需要更多的证据从核磁共振。结合NMR和MS / MS的调查，在四大SC-FUC酶解寡糖结构进行阐述fiNEfi艾德和在图4b所示。结果发现，那些四低聚糖的结构是非常相似的。组分II可以由分数我和nonsuphated岩藻糖残基。组分III包括分数和分数I II组分IV可以由两部分组分II。这些结果表明，disulphated trisfuose和disulphated tetrafucose可能占很大比例的海地瓜SC-FUC结构
骨干。同时，从海参、藻类等褐藻寡糖已报告了。陈等人。（2012）获得[ 3-a-l-fucp（2,4oso23）1？3-a-l-fucp -（2oso23）1？3-a-l -
FucP -（2oso23）- a-l-fucp-1？] n（n = 1,2）海参海参badionotus轻度酸水解。在这些低聚糖的比较，2-o-sulphation缺席在我们的低聚糖，这表明硫酸化岩藻聚糖硫酸酯的可能模式在不同种类的海参是不同的。许多研究人员认为，褐藻多糖硫酸酯寡糖从藻类中获得的是异质的各种糖的组合物，O-糖苷键组，分支（bakunina等人。，2002；碧兰等人。，2005；克莱门特等人。，2010；叶等人。，2005）。这些藻类与寡糖相比，棘皮动物寡糖更简单和规则的