The contractile or relaxant activit

The contractile or relaxant activities or both of aqueous extracts of green and roasted
coffees were assayed on isolated guinea pig tracheal spirals. Contractile and relaxant activities
were compared with histamine and theophylline, respectively. Green coffee extracts induced
concentration dependent contraction, but the maximal tension never exceeded 76-3% + 5-2 of a
maximal histamine contraction (0.69 + 0-07 g/mm2 v0-52 + 0.05 g/mm2; p < 0.01). One
gram of green coffee dust had a biological activity equivalent to 1.23 + 0-1 mg of histamine. The
pD2 value of histamine was -5-17 + 0-05. The potency of green coffee was unaffected by
mepyramine maleate (1 ,ug/ml, final bath concentration) while that of histamine was reduced 500
fold. Tissues contracted with histamine were not significantly relaxed by green coffee extracts. By
contrast, roasted coffee extracts induced concentration dependent relaxation of uncontracted and
histamine contracted tissues. Tissues contracted with green coffee extracts were also completely
relaxed by roasted coffee extracts. The pD, value of theophylline was -4-10 + 0-03. The relaxant
activity of 1 g of roasted coffee was equivalent to 1-95 + 0-16 mg of theophylline. The potency of
these extracts was significantly reduced after propranolol (1 gg/ml; dose ratio 1.56). Our results
show that coffee dust extracts have considerable biological activity which changes from a contractile
to a relaxant action as a consequence of processing. The greater incidence of adverse reactions
to green coffee dust(s) in coffee workers may be related to the contractile activity present in

結果 (中文) 1:

收缩或舒张活动或两个水浸液的绿色和烤咖啡测定对豚鼠离体气管螺旋状排列。收缩和舒张活动与对照组胺和茶碱，分别。绿咖啡提取物诱导浓度依赖的收缩，但最大张力从未超过 76 3%+ 5-2 的最大的组胺收缩 （0.69 + 0-07 g/mm2 v0 52 + 0.05 g/mm2 ； p < 0.01）。一个绿色咖啡尘埃克了生物活性等效到 1.23 + 0-1 毫克的组胺。的组胺 pD2 值是-5-17 + 0-05。咖啡生豆效价不受影响吡马来酸 （1，微克/毫升，最后浴浓度） 而组胺是减少的 500折叠。组织与组胺签约不明显放宽绿色咖啡提取物。由相反，烤咖啡提取物诱导浓度依赖性舒张的 uncontracted 和组胺收缩组织。绿色的咖啡提取物与签约的组织也是完全放宽烤咖啡提取物。PD 的茶碱价值是-4-10 + 0-03。松弛1 克的焙炒咖啡活性相当于 1 95 + 0-16 毫克小剂量茶碱。效力这些提取物显著降低后普萘洛尔 （1ml gg ； 剂量比 1.56）。我们的研究结果显示咖啡尘埃提取物具有相当大的生物活性的变化从收缩由于处理的松弛行动。更大的不良反应发生率向工人可能涉及收缩活动中存在的咖啡中的绿咖啡 dust(s)

結果 (中文) 3:

收缩或松弛的活动或是绿色和烤测定豚鼠离体气管螺旋
咖啡提取物。收缩性和舒张活动
与组胺和茶碱相比，分别。绿咖啡提取物诱导
浓度依赖性收缩，但最大张力不超过%的
76-3 5-2最大组胺收缩（0.69 0-07克/平方毫米v0-52 0.05克/平方毫米；P＜0.01）。一
克绿咖啡粉尘有生物活性相当于1.23毫克组胺0-1。组胺
PD2值- 5-17 0-05。通过
吡拉明马来酸绿咖啡的效力不受影响（1，μg/ml，最后浴浓度），组胺减少500
褶皱。组织收缩组胺没有显著放松绿咖啡萃取物。通过
对比，烤咖啡提取物诱导浓度依赖性松弛收缩和
组胺收缩组织。组织收缩，绿色咖啡提取物也完全
烤咖啡提取物放松。PD，价值茶碱是4-10 03。
松弛1克烤咖啡的活性相当于1-95 0-16毫克的茶碱。效力
这些提取物后，普萘洛尔显著降低（1克/毫升；剂量率1.56）。我们的研究结果表明，咖啡
灰尘提取物具有相当的生物活性的变化从一个收缩
到松弛作用的结果处理。不良反应的发生率较高
绿色的咖啡粉末（S）在咖啡工人可能存在的收缩活动相关