My thirst for knowledge is strong s

My thirst for knowledge is strong since I was young. I used to listen to my parents telling stories, and became curious about the natural world, thirsting for exploring it. When grew up, I was no longer content with those stories, so I began my own reading. I subscribed to We Love Science since elementary school, read lots of discoveries, wondering my own adventures someday. That’s the first time I had an interest in science.

Upon my entering Jilin University, I knew nothing about life science though. I chose to major in biotechnology just because I wanted to experience what I’ve read about in We Love Science when I was young. But at the end of my first semester, when I performed excellently in my academy and was given an opportunity to change my major, I hesitated. It was then I entered a class called Introduction to Life Science. During this general education course, the professor showed us the beauty and the charm of life science through his poetic description, passionate speech, vivid presentation. I was enchanted by this burgeoning field at once. When he explained to us how they finally managed to knockout some genes of the blue fox, changed its original white coat color, and decoded the cause of its death in ecstasy, I could feel his excitement, too, though I knew nothing about molecular genetics then. Through the course, I came to realize how wonderful life exists and brings about. At the end of this course, I made my decision to stay, for a keen fascination with life science. From then on, my journey was setting out to explore the colorful biology world.

Therefore, I entered the lab at the end of my freshman year. After several rotations, I decided to join the Host-Microbe Molecular Interaction Laboratory. The boss of the lab is professor Qin, a rigorous but gentle professor cared for each student he mentored. Our lab has a favorable academic atmosphere that everyone can make their achievements through assiduous work and good collaborations. While working in the lab, seniors are always helpful, and professor Qin would like to talk with us about our study or college life every day. I like the way the lab operates, people communicate, and the life moves on.

Later on, I got an opportunity to lead a team to perform our own research. This period of research taught me to be both a team leader and a team player. As our lab is doing research on pathogenesis of Botrytis cinerea, we often knockout some genes to see how it performs on plant leaves, then we complement those genes to retest its pathogenicity. But in fact, the result can be rather confused, for Botrytis cinerea is a kind of multinuclear fungus. So we decided to design an experiment to figure out how its nuclei distribute during mitosis. At first, we proposed a hypothesis based on the theses we referred to, that the bigger the cell is, the more the nuclei it will get during mitosis. To prove the hypothesis, we discussed with our tutor, and worked out a feasible plan. In our plan, we tried to insert two kinds of antibiotic marks into different nuclei within a specific spore of Botrytis cinerea, and use different kinds of resistant plates to select laboratory strains. Then we can subculture the strains to isolate monospore strains to do further research. I assigned tasks for everyone, hence we collaborated well to advance the process in an effective way. Everything got off the ground as I did excellent in proposal presentation and defense; we successfully designed and constructed two vectors, reorganized plasmids, transformed plasmids into agrobacterium, did preliminary experiment to determine the concentration of antibiotics. But after that, when it came to co-transformation, we really got into trouble. For a period of co-culture is about two weeks, and after two rounds of co-culture, we failed to obtain transformants. Then we analyzed the reason, did another co-culture. Again, we failed. We were puzzled and consulted our tutor for more advice. Under his instructions, my teammates and I then performed twenty-three rounds of co-culture, changing experiment conditions each time. We considered various pollutions, vitality of Botrytis cinerea, concentration of antibiotics, even changed G418 into chlorimuron-ethyl. But unfortunately, we failed all the experiments, sometimes we just got false positive transformants. That were definitely the gloomiest days we’d ever had during college life. I’d recall those days when I struggled to get out of my bed every morning, walked along the footpath to embrace the dark, bleak, winter crepuscule, only the Venus high hung, my footsteps echoed. Along the path stands a statue, with three rings around. I took that three rings as Hope, Courage and Life to me. Every time I passed it, strengths could gain from it to prop me up to be persistent. I was glad that my teammates could still be there to stand by me though they were also frustrated those days. The next summer vacation, I even stayed at the school.

結果 (中文) 1:

从我小时候起，我对知识的渴求是强烈。我过去常常听父母讲故事，和成为了自然的世界，充满好奇渴望探索它。当长大了，我不再是内容与那些故事，所以我开始我自己看书。我从小学开始订阅我们爱科学阅读了大量的发现，不知道如果有一天我自己的冒险经历。这是我第一次对科学的兴趣。在我进入的吉林大学，我也不知道生命科学虽然。我选择主修生物技术，只是因为我想要体验什么我读过关于在我们爱科学年轻时。但在结束了我的第一个学期，当我在我的学院表现出色，被给予机会来改变我的专业，我犹豫了一下。就在这时进入了一类被称为生命科学导论。此通识教育课程，教授向我们展示的美和生命科学通过他充满诗意的描写，热情洋溢的讲话，生动的演示文稿的魅力。我立刻被迷住了这一新兴领域。当他向我们解释如何他们最后管理到淘汰赛蓝狐狸一些基因，改变了其原始的白色毛色，和解码其死因在狂喜中的时，我能感觉自己兴奋的心情，太，虽然那时我知道分子遗传学一无所知。通过课程，我开始意识到如何精彩的人生存在，并且带来了。在本课程结束时，我作我决定留下来，为生命科学的热衷迷恋。从那时起，我的旅程着手探索丰富多彩的生物世界。因此，我在我的大一新生今年年底走进实验室。经过几轮调，我决定加入主机微生物分子相互作用实验室。劳顾会的老板是教授秦，严谨但温柔的教授照顾他指导每个学生。我们的实验室有一个良好的学术氛围，每个人都可以使他们的成就通过勤奋工作和良好的合作。在实验室里工作时，老人总是有用外教授秦想跟我们谈谈我们研究或大学的生活每一天。我喜欢劳顾会的方式运作，人们交流，和生活在前进。Later on, I got an opportunity to lead a team to perform our own research. This period of research taught me to be both a team leader and a team player. As our lab is doing research on pathogenesis of Botrytis cinerea, we often knockout some genes to see how it performs on plant leaves, then we complement those genes to retest its pathogenicity. But in fact, the result can be rather confused, for Botrytis cinerea is a kind of multinuclear fungus. So we decided to design an experiment to figure out how its nuclei distribute during mitosis. At first, we proposed a hypothesis based on the theses we referred to, that the bigger the cell is, the more the nuclei it will get during mitosis. To prove the hypothesis, we discussed with our tutor, and worked out a feasible plan. In our plan, we tried to insert two kinds of antibiotic marks into different nuclei within a specific spore of Botrytis cinerea, and use different kinds of resistant plates to select laboratory strains. Then we can subculture the strains to isolate monospore strains to do further research. I assigned tasks for everyone, hence we collaborated well to advance the process in an effective way. Everything got off the ground as I did excellent in proposal presentation and defense; we successfully designed and constructed two vectors, reorganized plasmids, transformed plasmids into agrobacterium, did preliminary experiment to determine the concentration of antibiotics. But after that, when it came to co-transformation, we really got into trouble. For a period of co-culture is about two weeks, and after two rounds of co-culture, we failed to obtain transformants. Then we analyzed the reason, did another co-culture. Again, we failed. We were puzzled and consulted our tutor for more advice. Under his instructions, my teammates and I then performed twenty-three rounds of co-culture, changing experiment conditions each time. We considered various pollutions, vitality of Botrytis cinerea, concentration of antibiotics, even changed G418 into chlorimuron-ethyl. But unfortunately, we failed all the experiments, sometimes we just got false positive transformants. That were definitely the gloomiest days we’d ever had during college life. I’d recall those days when I struggled to get out of my bed every morning, walked along the footpath to embrace the dark, bleak, winter crepuscule, only the Venus high hung, my footsteps echoed. Along the path stands a statue, with three rings around. I took that three rings as Hope, Courage and Life to me. Every time I passed it, strengths could gain from it to prop me up to be persistent. I was glad that my teammates could still be there to stand by me though they were also frustrated those days. The next summer vacation, I even stayed at the school.

結果 (中文) 2:

我对知识的渴求强烈，因为我还年轻。我曾经听我父母讲故事，并成为好奇的自然世界，渴望探索它。当长大后，我不再满足于那些故事，于是我开始了我自己的阅读。我订阅我们爱科学从小学，阅读了大量的发现，有一天我不知道自己的冒险。那是我第一次对科学的兴趣。

当我进入吉林大学，我也不知道，生命科学虽然。我选择主要在生物技术，只是因为我想体验我读过关于我们爱科学，当我还年轻。但在我的第一个学期，当我在学院进行的出色，并有机会来改变我的专业的结尾，我犹豫了。就在那时我进入了一个叫做介绍生命科学类。在此通识教育课程，教授向我们展示了美丽与生命科学，通过他的诗歌描述的魅力，激情演讲，生动的介绍。我被这个新兴领域的一次陶醉。当他向我们解释他们是如何最终设法淘汰赛蓝狐的某些基因，改变了原来的白色毛色，并解码其在狂喜的死因，我能感觉到他的兴奋，太，虽然我也不知道，分子遗传学然后。通过过程中，我逐渐认识到生活是多么美好的存在，带来了。在这个课程结束后，我做了我的决定留下来，与生命科学产生了浓厚的魅力。从此，我的旅程出发探索丰富多彩的生物世界。

因此，我进入了实验室在我大一结束。几个旋转后，我决定加入主机微生物分子相互作用实验室。该实验室的老板是秦教授，严谨而温柔的教授照顾他辅导每一位学生。我们实验室有一个良好的学术氛围，每个人都可以通过刻苦的工作和良好的合作他们的成就。而在实验室工作的，老人总是有益的，和秦教授希望与我们谈论我们的学习或大学生活的每一天。我喜欢实验室的运作方式，人们的沟通和生活在移动。

后来，我有机会带领一个团队来执行我们自己的研究。研究这一时期教我既当领队和团队合作精神。由于我们的实验室正在做灰霉病的发病机制的研究中，我们经常敲除某些基因，看看它是如何执行的植物叶片，然后我们补充这些基因重新测试它的致病性。但实际上，结果可能是比较混乱，对灰霉病是一种多核真菌。因此，我们决定设计一个实验来找出它的原子核分裂过程中是如何分布。首先，我们提出了基于我们所提到的论文一个假设，即细胞越大，越原子核就会有丝分裂过程得到。为了证明这一假设，我们与导师讨论，并制定出了切实可行的计划。在我们的计划中，我们试图灰霉病的特定孢子内插入两个种抗生素标记成不同的原子核，并使用不同种类的抗板块选择实验室菌株。然后我们就可以传代培养菌株分离菌株单孢做进一步的研究。我分配任务给大家，因此我们合作很好地推动这一进程的有效途径。每样东西掉在地上，像我一样优秀的提案陈述和申辩; 我们成功设计并构建了两个载体，重组质粒，转化质粒导入农杆菌，做过初步的实验，以确定抗生素的浓度。但在那之后，当它来到共转化，我们真的遇到了麻烦。一段共培养约两个星期，经过两轮共培养，我们未能获得转化体。然后，我们分析了原因，却是另一位联合培养。同样，我们失败了。我们疑惑地咨询我们的导师更多的建议。根据他的指示，我和我的队友，然后进行23轮共培养，每一次变化的实验条件。我们考虑了各种污染，灰霉病活力，抗生素浓度，甚至改变了G418为氯嘧磺隆。但不幸的是，我们失败了所有的实验，有时我们刚拿到假阳性转化。这是肯定我们会大学生活中曾经有过的最悲观的日子。我回想起那些日子，我挣扎着每天早上走出我的床，沿着人行道走到拥抱黑暗，萧瑟，冬crepuscule，只有金星高鸿，我的脚步声回荡。沿着小路矗立着一座雕像，与围绕三个环。我把那三个环的希望，勇气和生命给我。我每次经过它，优势可以从中获得支撑我是持久的。我很高兴我的队友们仍然可以通过有我站在尽管他们也感到沮丧的日子。接下来的暑假，我甚至住在学校。

結果 (中文) 3:

我对知识的渴望是强大的，因为我是年轻的。我曾经听我的父母讲故事，成为对自然世界的好奇，渴望探索它。当长大了，我不再满足那些故事，所以我开始我自己的阅读。我订阅了我们爱的科学，从小学，阅读大量的发现，想知道我自己的冒险有一天。这是我第一次对科学感兴趣。当我进入吉林大学时，我对生命科学一无所知。我选择主修生物技术，只是因为我想体验我读过的关于在我们爱科学的时候，我年轻的时候。但在我的第一个学期结束，当我的表现非常出色，在我院获得了一个机会去改变我的专业，我犹豫了。然后我进入了一个叫做生命科学的入门班。在这通识教育的过程中，通过他的诗意描述、激情演讲、生动呈现，展示了生命科学的美和魅力。我一下子被这个新兴的领域迷住了。当他向我们解释他们是如何最终成功地淘汰了蓝狐的一些基因，改变了它原来的白色外套的颜色，并解码其死亡的原因，在狂喜，我也可以感觉到他的兴奋，虽然我不知道分子遗传学。通过这一过程，我开始意识到生活是多么美好，并带来了。在本课程结束时，我决定留下来，对生命科学有着浓厚的吸引力。从那时起，我的旅程开始探索多彩的生物世界。因此，我在大学一年级时就进入了实验室。经过几次旋转，我决定加入宿主微生物分子相互作用实验室。实验室的老板是秦教授，一个严谨而温柔的关心每一个学生他指导教授。我们实验室有一个良好的学术氛围，每个人都可以使他们的成果通过勤奋的工作和良好的合作关系。在实验室里工作的时候，老年人总是乐于助人，秦教授每天都想和我们谈谈我们的学习或大学生活。我喜欢实验室的运作方式，人们交流，生活的节奏。后来，我有机会领导一个团队来执行我们自己的研究。这一时期的研究告诉我，无论是一个团队的领导者和一个团队的球员。我们的实验室正在对番茄灰霉病的发病机制的研究，我们经常看到一些基因敲除的表现如何对植物的叶子，然后补充那些复试的致病基因。但事实上，其结果是非常混乱的，Botrytis cinerea是一种多核菌。因此，我们决定设计一个实验来确定它的细胞核在有丝分裂过程中的分布。首先，我们提出了一个假设的基础上，我们所指的，即越大的细胞，在有丝分裂过程中，它会得到更多的核。为了证明这一假设，我们与我们的导师讨论，并制定了一个可行的计划。在我们的计划中，我们试图将两种抗生素标记为不同的核在一个特定的孢子灰霉病，使用不同种类的抗板实验室菌株的选择。然后我们可以传代菌株分离单孢菌株做进一步的研究。我为每个人分配任务，因此，我们很好地合作，以有效的方式推进这一进程。在建议的演示和防御中，一切都离地面，我们成功地设计和构建了两个载体，重组质粒，转化质粒转化为根癌农杆菌，做了初步实验，以确定抗生素的浓度。但在那之后，当它来到共同转型，我们真的陷入了麻烦。一段共培养约两周，并经过两轮共培养，我们未能获得转化子。然后我们分析了原因，做了另一种共同文化。再次，我们失败了。我们感到困惑，并咨询我们的导师，征求更多的意见。在他的指导下，我的队友和我然后进行了二十三轮共培养，改变实验条件下，每一次。我们考虑了各种污染，灰葡萄孢菌的活力，浓度的抗生素，甚至改变了G418为氯嘧磺隆。但不幸的是，我们没有所有的实验中，有时候我们只是假阳性转化子。那肯定是在大学生活最黑暗的日子里我们会。我想起那些日子，当我挣扎着想出来，每天早上我的床上，沿着人行道拥抱黑暗，阴冷的冬天的黄昏，只有金星高挂，我的脚步声。沿着小路矗立着一尊雕像，周围有三个戒指。我把那三枚戒指作为希望，勇气和生命给我。每一次我通过它，优势可以从它获得，以支持我的持久性。我很高兴，我的队友们仍然可以站在我身边，虽然他们也感到沮丧的那些日子。下一个暑假，我甚至呆在学校里。