PDEnRich™ is derived from a silica-based NuGel™ multi-dimensional surface library and optimized for the enrichment of phosphodiesterase activity. The standard prep protocol starts with 100 μl of tissue/cell extracts, or approximately 0.5 mg total protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. The kit includes all necessary reagents for immediate use.