The phenomenex C-18 column was equilibrated with the mobile phase, acetonitrile: 10 mM potassium dihydrogen phosphate15:85 (v/v); pH 2.5. <br><br>The active drug was monitored with UV detector at 210 nm, and the injection volume was 50 μl. The flow rate was maintained at 1.0 ml/min and the total run time was kept 8 min.
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