4.3.2 SETTLER PERFORMANCE
4.3.2.1 Settlement success
Settlement success was strongly affected by concentration of copper and patterns of variation were significantly different with the source of copper (two-way ANOVA;Source: F(2,24) = 11.4, p < 0.001; Copper level: F(2,24) = 53.7, p < 0.001; Interaction term: F(6,24) = 16.2, p < 0.001; Figure 4.5). Eight days after having been placed in settlement containers, significantly less urchins had settled successfully in the ANZECC and High Pulse levels in the Diet treatment, and across all copper levels in the Diet + Water treatments (Tukey HSD, p < 0.05; Figure 4.5). The High Pulse level had a stronger impact than the High Chronic level in the Diet and the Diet + Water treatments, with almost no settlement at the High Pulse level. In the Water treatment, only the High Chronic level was significantly different from controls, with 3.6% settlement success. The best model included no covariates and model fit was
excellent, with a 92% multiple R2.