Moreover,
0.1 mM NADPH was added to the vials before counting to assess the activation
of NADPH oxidase activity in the samples. Basal superoxide-induced luminescence
was then subtracted from the luminescence value induced by NADPH
[29]. To confirm the involvement of NADPH oxidase, diphenyleneiodonium
(DPI, 10 mmol/L), a selective inhibitor of NADPH oxidase was used according to
a previous study [