After centrifugation, the supernata

After centrifugation, the supernatant was desalted by non-por-ous graphitised carbon black column as previously reported (Packer, Lawson, Jardine, & Redmond, 1998). In brief, oligosaccharide solutions were applied to a Carbograph SPE column (SUPELCO, USA). Salts were washed with approximately three column volumes of water. The oligosaccharides were adsorbed and then
eluted with 25% (v/v) acetonitrile in 0.05% (v/v) TFA, which was collected, concentrated and lyophilised.

結果 (中文) 1:

离心后上清液被淡化按非 por ou graphitised 炭黑列如以前所报告 (封隔器、 劳森、 怡和，雷德蒙德，1998年)。简单地说，低聚糖的解决方案适用于 Carbograph SPE 柱 (其中的水蒸气、 USA)。盐被用大约三个柱体积的水冲走。低聚糖被吸附，然后在 0.05%（v/v） 反式脂肪酸，这是收集、 集中和冻干的 25%（v/v） 乙腈洗脱。

結果 (中文) 3:

离心后，上清液淡化非空隙性石墨化炭黑柱如先前报道（封隔器，劳森，怡和，&雷德蒙德，1998）。总之，寡糖的解决方案被应用于一个Carbograph固相萃取柱（Supelco公司，美国）。盐与水约三的柱体积洗。寡糖吸附然后
用25%（v/v）乙腈0.05%（v/v）TFA，这是收集，浓缩，冷冻干燥。