The most successful research using

The most successful research using adventitious roots for cosmetics ingredients is saikosaponin production by
root culture of B. falcatum L. The root of B. falcatum L, known as Bupleuri radix, is a galenical formulated in a variety
of TCMs. Among the more than 10 different saikosaponins, SSa and SSd are especially known as pharmacologically
active components, possessing properties such as antiallergic activity, analgesic action, and antiinflammatory
action.44–46 SSb1 and SSb2, which are produced artificially from SSa and SSd, have been recognized
as unique biologically active substances for skin cells, as described in Section 3.11.5.47–49 Kusakari et al.50 overcame
the defect of slower growth of adventitious roots by regulating lateral root differentiation. They found that the
formation of the lateral roots, which was induced in the presence of auxin (indolebutyric acid), was strongly suppressed as the sugar concentration was increased (Figure 32). This effect may be involved in the scavenging
effect of sugar on hydroxyl radical51 because lateral root initiation in B. falcatum root culture was promoted by
stresses such as drought or heat, and active oxygen species that the addition of hydrogen peroxide or methylviologen
contributed.52 As saikosaponin was accumulated only on lateral roots and original roots were losing their
function to root tissues during the culture in that culture system, the prompt increase of lateral root formation was
crucially important. However, as sucrose is also an energy source, a two-step culture was adopted, with the addition
of 1% sucrose at the beginning of the culture and 6%sucrose thereafter at 14 days when lateral roots had emerged.
This means adding sugar greatly improved the productivity, affording 0.8 g l1 of SSa and SSd. In addition, they
developed a new type of tank for commercial production (Figure 33) and have been producing the extract
containing SSb1 and SSb2, which were produced by converting SSa and SSd in the extract by regulating pH.
Saikosaponin-containing extract was compounded in a new brand of cosmetics called ‘Bioperformance’ by Shiseido
Co., Ltd., successfully marketed in Europe. Saikosaponin-containing extract has been used in cosmetics for more
than 10 years; this extract was the first repeat product manufactured by biotechnological means.

結果 (繁體中文) 1:

The most successful research using adventitious roots for cosmetics ingredients is saikosaponin production byroot culture of B. falcatum L. The root of B. falcatum L, known as Bupleuri radix, is a galenical formulated in a varietyof TCMs. Among the more than 10 different saikosaponins, SSa and SSd are especially known as pharmacologicallyactive components, possessing properties such as antiallergic activity, analgesic action, and antiinflammatoryaction.44–46 SSb1 and SSb2, which are produced artificially from SSa and SSd, have been recognizedas unique biologically active substances for skin cells, as described in Section 3.11.5.47–49 Kusakari et al.50 overcamethe defect of slower growth of adventitious roots by regulating lateral root differentiation. They found that theformation of the lateral roots, which was induced in the presence of auxin (indolebutyric acid), was strongly suppressed as the sugar concentration was increased (Figure 32). This effect may be involved in the scavengingeffect of sugar on hydroxyl radical51 because lateral root initiation in B. falcatum root culture was promoted bystresses such as drought or heat, and active oxygen species that the addition of hydrogen peroxide or methylviologencontributed.52 As saikosaponin was accumulated only on lateral roots and original roots were losing theirfunction to root tissues during the culture in that culture system, the prompt increase of lateral root formation was極為重要。然而，經蔗糖也是一個能量源，兩步文化獲得通過，加上1%的出現了蔗糖在此後第 14 天當側根開始的文化和 6%的蔗糖。這意味著添加糖大大提高了生產率，提供 0.8 g l 1 的 SSa 和 SSd。此外，他們開發一種新型的坦克為商業化生產 (圖 33)，一直在生產提取物含 SSb1 和 SSb2，產生的轉換 SSa 和 SSd 提取物中，通過調節 ph 值。含柴胡皂苷的提取劑複配，叫做 'Bioperformance' 的資生堂化妝品新品牌股份有限公司，成功地在歐洲市場銷售。含柴胡皂苷的提取物已被用於化妝品中更多超過 10 年;這種提取物是通過生物技術手段製造的第一次重複產品。

結果 (繁體中文) 2:

使用不定根化妝品成分最成功的研究是由柴胡皂苷生產
B. falcatum屬植物B. falcatum L，被稱為柴胡根的根文化，是在多種制定了草藥
中藥的。其中超過10種不同的皂苷，SSA和SSD是特別被稱為藥理
活性成分，具有如抗過敏作用，鎮痛作用，抗炎和性能
action.44-46 SSB1和SSB2，這是從SSA和SSD人工生產的，有被認定
為第描述為獨特的生物活性物質，對皮膚細胞，3.11.5.47-49草刈等AL.50克服
通過調節側根分化不定根生長較慢的缺陷。他們發現，
側根，這是誘導生長素（吲哚丁酸）的存在下形成的，被強烈的抑制作為糖濃度增加（圖32）。可能涉及的清除這種效果
的糖上的羥基radical51影響，因為在B中falcatum根培養側根開始被提升由
逆境如乾旱或熱，並且活性氧種，添加過氧化氫或甲基紫
contributed.52作為柴胡皂苷積累只在側根和原有的根系失去他們
在培養體系培養中功能根部組織，側根形成迅速增長是
至關重要的。然而，如蔗糖也是能量源，兩個步驟的培養物通過，通過加入
在培養和6％蔗糖的其後當側根出現了14天開始1％的蔗糖。
這意味著大大加入糖提高了工作效率，得到0.8 GL？1 SSA和SSD的。此外，他們還
開發了一種新型坦克的商業化生產（圖33），並一直在生產提取物
含有SSB1和SSB2，這是通過將SSA和SSD的提取物通過調節pH值產生的。
柴胡皂甙含提取物混合在化妝品新品牌名為“生物性能”資生堂
有限公司，成功地在歐洲銷售。柴胡皂甙含提取物已在化妝品中為多被使用
超過10年; 該提取物是通過生物技術手段製造的第一重複產物。

結果 (繁體中文) 3:

最成功的研究利用不定根的化妝品成分是由
對三島柴胡L.三島柴胡l根文化生產柴胡皂甙，稱為柴胡，是一種草藥在中藥品種
製定。超過10種不同的柴胡總皂苷中，SSA與SSD特別稱為活性成分的藥理
，具有效能如抗過敏活性，鎮痛作用，抗炎
行動。44–46 SSB1及SSB2、這是人工製造的SSA和SSD，已被公認
作為皮膚細胞獨特的生物活性物質，如部分3.11.5.47–49 kusakari等al.50克服
調節側根分化對不定根生長緩慢的缺陷。他們發現
側根的形成，這是生長素誘導下（吲哚丁酸），强烈抑制糖濃度的新增（圖32）。這種影響可能參與了清除