Leaves of the plant species listed in Table 1, including ohia, were inoculated by placing a 10-ll drop of a urediniospore suspension(10 spores/ml) on their surface. The inoculated plants were placed in a clear plastic box maintained at 100% humidity and 22°C in the dark for 24 h, then transferred to a growth chamber at 22°C with a 12-h photoperiod for
further observation.