The igg elisa kit technical principle
With the wide application of the igg elisa kit technology in the fields of medicine and biology, in vitro detection of various cytokines and antibody research a new breakthrough. Been commonly used in the study of the immune response mechanism by enzyme-linked immunosorbent assay (ELISA) in the free cytokines (CK), or antibody in body fluids, but because of the different semi-Isles of free circulating antibody or CK, so that in the body fluids constantly metabolism or binding to target organs, and can not exactly reflect the bodys antibodies and CK levels. 80s according to the basic principle of the ELISA technique, the foreign research workers established in vitro detection of specific antibody-secreting cells and CK secreting cells in solid phase igg elisa kit. Because of its high specificity and sensitivity, is being widely in applications of great significance to explore the pathogenesis of autoimmune diseases.
The igg elisa kit method from ELISA, and break through the traditional ELISA method is an extension of the quantitative ELISA techniques and new developments. Both detect cells to produce cytokines or other soluble proteins, the biggest difference is that:
1, The igg elisa kit in color reaction in the microplate absorbance was measured with a standard curve comparing the total soluble protein.
2, The igg elisa kit also in color reaction can show a clearly discernible spots on the corresponding location of the cells secrete soluble protein, can be directly under the microscope manual counting of spots or by CTL ELISA analysis system to count the spots, a the spot represents a cell, thereby calculating the frequency of the cells to secrete the protein. (Some research is not only to measure the amount of cytokine production, cell frequencies needed to detect this cytokine secretion).