Liaquat and Apenten (2000) studied the
formation of low molecular weight esters in an
organic medium composed of hexane, using
enzymes precipitated from different vegetable seeds
by ammonium sulphate. Direct esterification of
acetic, butyric and caproic acid by ethanol, butanol,
isobutanol or (Z)-3-hexen-1-ol was also carried out.
Of the grain lipases studied (wheat, linseed, barley
and canola), canola seed lipase showed the highest
degree of flavor formation, producing (Z)-3-hexen-1-
yl butyrate and (Z)-3-hexen-1-yl caproate with an
efficiency of about 96%.